Community

• Community Standards
• Lab Notebooks/Data Management
• The “If I were a leave-nasty-notes-in-the-lab-person, I would write…” Page

protocols

Molecular Biology

• Normal cloning
• LIC cloning
• Site-directed mutagenesis

Protein Expression/Purification

• Basic bacterial growth/induction protocol
• Using the FPLC
• S100A2-A6 current prep protocol
• S100A8/A9 pETDuet Heterodimer Prep

Protein Characterization

• Sample preparation and quality assurance
• Measuring absorbance
• Circular Dichroism spectroscopy
• Isothermal Titration Calorimetry
• Lore for keeping transition metals happy

Phage Display

• Cloning into T7Select vector arms
• Prepping phage for a binding experiment

Bacterial Surface Display

• Outer membrane protein extraction

Lab Stocks

• Stock solutions
• Lab chemical inventory
• TEV protease